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atf3 protein levels  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology atf3 protein levels
    Fig. 1 Analysis of fluorescence intensity in HeLa and Ca Ski cells transfected with <t>pCMV6-ATF3,</t> a mock vector, or left untreated as controls, utilizing fluorescence microscopy and flow cytometry at 48 and 72 h post-transfection. (A) Fluorescent imaging of HeLa and Ca Ski cells was performed at 20x magnification, comparing cells transfected with pCMV6-ATF3 to mock and control groups. (B) Mean fluorescence intensity levels were measured across pCMV6-ATF3, mock, and control samples. Flow cytometry results showed transfection efficiencies of 89.41% in HeLa and 88.13% in Ca Ski cells at 72 h post-transfection, significantly higher than the mock and control groups (p < 0.0001). (C) Western blot analysis was conducted to assess ATF3 protein ex pression in HeLa and Ca Ski cell lysates, using anti-ATF3 and anti-β-actin antibodies. (D) Densitometry of ATF3 expression levels showed a marked increase in cells transfected with pCMV6-ATF3 compared to control and mock groups (p < 0.001). Results are presented as the mean ± SD from three independent experiments
    Atf3 Protein Levels, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 636 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/atf3+protein+levels/pm39994644-79-0-11?v=Santa+Cruz+Biotechnology
    Average 94 stars, based on 636 article reviews
    atf3 protein levels - by Bioz Stars, 2026-07
    94/100 stars

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    1) Product Images from "Activating transcription factor 3 induces oxidative stress and genotoxicity, transcriptionally modulating metastasis-related gene expression in human papillomavirus-infected cervical cancer."

    Article Title: Activating transcription factor 3 induces oxidative stress and genotoxicity, transcriptionally modulating metastasis-related gene expression in human papillomavirus-infected cervical cancer.

    Journal: Virology journal

    doi: 10.1186/s12985-025-02675-0

    Fig. 1 Analysis of fluorescence intensity in HeLa and Ca Ski cells transfected with pCMV6-ATF3, a mock vector, or left untreated as controls, utilizing fluorescence microscopy and flow cytometry at 48 and 72 h post-transfection. (A) Fluorescent imaging of HeLa and Ca Ski cells was performed at 20x magnification, comparing cells transfected with pCMV6-ATF3 to mock and control groups. (B) Mean fluorescence intensity levels were measured across pCMV6-ATF3, mock, and control samples. Flow cytometry results showed transfection efficiencies of 89.41% in HeLa and 88.13% in Ca Ski cells at 72 h post-transfection, significantly higher than the mock and control groups (p < 0.0001). (C) Western blot analysis was conducted to assess ATF3 protein ex pression in HeLa and Ca Ski cell lysates, using anti-ATF3 and anti-β-actin antibodies. (D) Densitometry of ATF3 expression levels showed a marked increase in cells transfected with pCMV6-ATF3 compared to control and mock groups (p < 0.001). Results are presented as the mean ± SD from three independent experiments
    Figure Legend Snippet: Fig. 1 Analysis of fluorescence intensity in HeLa and Ca Ski cells transfected with pCMV6-ATF3, a mock vector, or left untreated as controls, utilizing fluorescence microscopy and flow cytometry at 48 and 72 h post-transfection. (A) Fluorescent imaging of HeLa and Ca Ski cells was performed at 20x magnification, comparing cells transfected with pCMV6-ATF3 to mock and control groups. (B) Mean fluorescence intensity levels were measured across pCMV6-ATF3, mock, and control samples. Flow cytometry results showed transfection efficiencies of 89.41% in HeLa and 88.13% in Ca Ski cells at 72 h post-transfection, significantly higher than the mock and control groups (p < 0.0001). (C) Western blot analysis was conducted to assess ATF3 protein ex pression in HeLa and Ca Ski cell lysates, using anti-ATF3 and anti-β-actin antibodies. (D) Densitometry of ATF3 expression levels showed a marked increase in cells transfected with pCMV6-ATF3 compared to control and mock groups (p < 0.001). Results are presented as the mean ± SD from three independent experiments

    Techniques Used: Fluorescence, Transfection, Plasmid Preparation, Microscopy, Flow Cytometry, Imaging, Control, Western Blot, Expressing

    Fig. 2 Effect of ATF3 overexpression on the expression of HPV-16 and HPV-18 E6/E7, MMP1, and SHARP1 genes in HeLa (A) and Ca Ski (B) cells, analyzed by RT-qPCR. (A) In HeLa cells, overexpression of ATF3 resulted in a significant decrease in MMP1 gene expression (p < 0.01), while SHARP1 and HPV-18 E6/ E7 mRNA levels remained largely unaffected (p > 0.05) compared to mock and control groups. (B) Similarly, in Ca Ski cells, ATF3 overexpression significantly reduced MMP1 expression (p < 0.02), but SHARP1 and HPV-18 E6/E7 expression levels showed no significant difference (p > 0.05) relative to mock and control groups. Both HeLa and Ca Ski cells transfected with pCMV6-ATF3 demonstrated markedly higher ATF3 mRNA expression compared to mock and control groups (p < 0.001). Data are presented as the mean ± SD from three independent experiments
    Figure Legend Snippet: Fig. 2 Effect of ATF3 overexpression on the expression of HPV-16 and HPV-18 E6/E7, MMP1, and SHARP1 genes in HeLa (A) and Ca Ski (B) cells, analyzed by RT-qPCR. (A) In HeLa cells, overexpression of ATF3 resulted in a significant decrease in MMP1 gene expression (p < 0.01), while SHARP1 and HPV-18 E6/ E7 mRNA levels remained largely unaffected (p > 0.05) compared to mock and control groups. (B) Similarly, in Ca Ski cells, ATF3 overexpression significantly reduced MMP1 expression (p < 0.02), but SHARP1 and HPV-18 E6/E7 expression levels showed no significant difference (p > 0.05) relative to mock and control groups. Both HeLa and Ca Ski cells transfected with pCMV6-ATF3 demonstrated markedly higher ATF3 mRNA expression compared to mock and control groups (p < 0.001). Data are presented as the mean ± SD from three independent experiments

    Techniques Used: Over Expression, Expressing, Quantitative RT-PCR, Gene Expression, Control, Transfection

    Fig. 3 Evaluation of ROS levels in HeLa and Ca Ski cells following pCMV6-ATF3 transfection. (A) Flow cytometry histograms illustrating ROS levels in HeLa and Ca Ski cells, measured with the DCHF-DA assay. (B) Quantitative comparison shows a significant rise in ROS levels in pCMV6-ATF3-transfected cells relative to mock and control groups (p < 0.01). Results are shown as the mean ± SD from three separate experiments
    Figure Legend Snippet: Fig. 3 Evaluation of ROS levels in HeLa and Ca Ski cells following pCMV6-ATF3 transfection. (A) Flow cytometry histograms illustrating ROS levels in HeLa and Ca Ski cells, measured with the DCHF-DA assay. (B) Quantitative comparison shows a significant rise in ROS levels in pCMV6-ATF3-transfected cells relative to mock and control groups (p < 0.01). Results are shown as the mean ± SD from three separate experiments

    Techniques Used: Transfection, Flow Cytometry, Comparison, Control

    Fig. 4 DNA damage analysis by comet assay using alkaline single-cell gel electrophoresis. (A) Representative images from the comet assay display DNA migration patterns in HeLa and Ca Ski cells post-transfection with pCMV6-ATF3. In these images, intact DNA forms a distinct head, while fragmented DNA trails behind, forming a comet tail—longer tails signify greater DNA damage. (B) Quantification of comet assay results for HeLa and Ca Ski cells across pCMV6-ATF3-transfected, mock-transfected, and control groups. Minimal DNA damage was observed in both control and mock-transfected cells. Data are expressed as the mean ± SD from three independent replicates
    Figure Legend Snippet: Fig. 4 DNA damage analysis by comet assay using alkaline single-cell gel electrophoresis. (A) Representative images from the comet assay display DNA migration patterns in HeLa and Ca Ski cells post-transfection with pCMV6-ATF3. In these images, intact DNA forms a distinct head, while fragmented DNA trails behind, forming a comet tail—longer tails signify greater DNA damage. (B) Quantification of comet assay results for HeLa and Ca Ski cells across pCMV6-ATF3-transfected, mock-transfected, and control groups. Minimal DNA damage was observed in both control and mock-transfected cells. Data are expressed as the mean ± SD from three independent replicates

    Techniques Used: Single Cell Gel Electrophoresis, Alkaline Single Cell Gel Electrophoresis, Migration, Transfection, Control



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    atf3 protein levels  (Santa Cruz Biotechnology)
    94
    Santa Cruz Biotechnology atf3 protein levels
    Fig. 1 Analysis of fluorescence intensity in HeLa and Ca Ski cells transfected with <t>pCMV6-ATF3,</t> a mock vector, or left untreated as controls, utilizing fluorescence microscopy and flow cytometry at 48 and 72 h post-transfection. (A) Fluorescent imaging of HeLa and Ca Ski cells was performed at 20x magnification, comparing cells transfected with pCMV6-ATF3 to mock and control groups. (B) Mean fluorescence intensity levels were measured across pCMV6-ATF3, mock, and control samples. Flow cytometry results showed transfection efficiencies of 89.41% in HeLa and 88.13% in Ca Ski cells at 72 h post-transfection, significantly higher than the mock and control groups (p < 0.0001). (C) Western blot analysis was conducted to assess ATF3 protein ex pression in HeLa and Ca Ski cell lysates, using anti-ATF3 and anti-β-actin antibodies. (D) Densitometry of ATF3 expression levels showed a marked increase in cells transfected with pCMV6-ATF3 compared to control and mock groups (p < 0.001). Results are presented as the mean ± SD from three independent experiments
    Atf3 Protein Levels, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/atf3+protein+levels/pm39994644-79-0-11?v=Santa+Cruz+Biotechnology
    Average 94 stars, based on 1 article reviews
    atf3 protein levels - by Bioz Stars, 2026-07
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Fig. 1 Analysis of fluorescence intensity in HeLa and Ca Ski cells transfected with pCMV6-ATF3, a mock vector, or left untreated as controls, utilizing fluorescence microscopy and flow cytometry at 48 and 72 h post-transfection. (A) Fluorescent imaging of HeLa and Ca Ski cells was performed at 20x magnification, comparing cells transfected with pCMV6-ATF3 to mock and control groups. (B) Mean fluorescence intensity levels were measured across pCMV6-ATF3, mock, and control samples. Flow cytometry results showed transfection efficiencies of 89.41% in HeLa and 88.13% in Ca Ski cells at 72 h post-transfection, significantly higher than the mock and control groups (p < 0.0001). (C) Western blot analysis was conducted to assess ATF3 protein ex pression in HeLa and Ca Ski cell lysates, using anti-ATF3 and anti-β-actin antibodies. (D) Densitometry of ATF3 expression levels showed a marked increase in cells transfected with pCMV6-ATF3 compared to control and mock groups (p < 0.001). Results are presented as the mean ± SD from three independent experiments

    Journal: Virology journal

    Article Title: Activating transcription factor 3 induces oxidative stress and genotoxicity, transcriptionally modulating metastasis-related gene expression in human papillomavirus-infected cervical cancer.

    doi: 10.1186/s12985-025-02675-0

    Figure Lengend Snippet: Fig. 1 Analysis of fluorescence intensity in HeLa and Ca Ski cells transfected with pCMV6-ATF3, a mock vector, or left untreated as controls, utilizing fluorescence microscopy and flow cytometry at 48 and 72 h post-transfection. (A) Fluorescent imaging of HeLa and Ca Ski cells was performed at 20x magnification, comparing cells transfected with pCMV6-ATF3 to mock and control groups. (B) Mean fluorescence intensity levels were measured across pCMV6-ATF3, mock, and control samples. Flow cytometry results showed transfection efficiencies of 89.41% in HeLa and 88.13% in Ca Ski cells at 72 h post-transfection, significantly higher than the mock and control groups (p < 0.0001). (C) Western blot analysis was conducted to assess ATF3 protein ex pression in HeLa and Ca Ski cell lysates, using anti-ATF3 and anti-β-actin antibodies. (D) Densitometry of ATF3 expression levels showed a marked increase in cells transfected with pCMV6-ATF3 compared to control and mock groups (p < 0.001). Results are presented as the mean ± SD from three independent experiments

    Article Snippet: ATF3 protein levels were detected using a monoclonal anti-ATF3 antibody (1:500, Santa Cruz Biotechnologies). β-actin (1:5000, Santa Cruz Biotechnologies) was used as a loading control.

    Techniques: Fluorescence, Transfection, Plasmid Preparation, Microscopy, Flow Cytometry, Imaging, Control, Western Blot, Expressing

    Fig. 2 Effect of ATF3 overexpression on the expression of HPV-16 and HPV-18 E6/E7, MMP1, and SHARP1 genes in HeLa (A) and Ca Ski (B) cells, analyzed by RT-qPCR. (A) In HeLa cells, overexpression of ATF3 resulted in a significant decrease in MMP1 gene expression (p < 0.01), while SHARP1 and HPV-18 E6/ E7 mRNA levels remained largely unaffected (p > 0.05) compared to mock and control groups. (B) Similarly, in Ca Ski cells, ATF3 overexpression significantly reduced MMP1 expression (p < 0.02), but SHARP1 and HPV-18 E6/E7 expression levels showed no significant difference (p > 0.05) relative to mock and control groups. Both HeLa and Ca Ski cells transfected with pCMV6-ATF3 demonstrated markedly higher ATF3 mRNA expression compared to mock and control groups (p < 0.001). Data are presented as the mean ± SD from three independent experiments

    Journal: Virology journal

    Article Title: Activating transcription factor 3 induces oxidative stress and genotoxicity, transcriptionally modulating metastasis-related gene expression in human papillomavirus-infected cervical cancer.

    doi: 10.1186/s12985-025-02675-0

    Figure Lengend Snippet: Fig. 2 Effect of ATF3 overexpression on the expression of HPV-16 and HPV-18 E6/E7, MMP1, and SHARP1 genes in HeLa (A) and Ca Ski (B) cells, analyzed by RT-qPCR. (A) In HeLa cells, overexpression of ATF3 resulted in a significant decrease in MMP1 gene expression (p < 0.01), while SHARP1 and HPV-18 E6/ E7 mRNA levels remained largely unaffected (p > 0.05) compared to mock and control groups. (B) Similarly, in Ca Ski cells, ATF3 overexpression significantly reduced MMP1 expression (p < 0.02), but SHARP1 and HPV-18 E6/E7 expression levels showed no significant difference (p > 0.05) relative to mock and control groups. Both HeLa and Ca Ski cells transfected with pCMV6-ATF3 demonstrated markedly higher ATF3 mRNA expression compared to mock and control groups (p < 0.001). Data are presented as the mean ± SD from three independent experiments

    Article Snippet: ATF3 protein levels were detected using a monoclonal anti-ATF3 antibody (1:500, Santa Cruz Biotechnologies). β-actin (1:5000, Santa Cruz Biotechnologies) was used as a loading control.

    Techniques: Over Expression, Expressing, Quantitative RT-PCR, Gene Expression, Control, Transfection

    Fig. 3 Evaluation of ROS levels in HeLa and Ca Ski cells following pCMV6-ATF3 transfection. (A) Flow cytometry histograms illustrating ROS levels in HeLa and Ca Ski cells, measured with the DCHF-DA assay. (B) Quantitative comparison shows a significant rise in ROS levels in pCMV6-ATF3-transfected cells relative to mock and control groups (p < 0.01). Results are shown as the mean ± SD from three separate experiments

    Journal: Virology journal

    Article Title: Activating transcription factor 3 induces oxidative stress and genotoxicity, transcriptionally modulating metastasis-related gene expression in human papillomavirus-infected cervical cancer.

    doi: 10.1186/s12985-025-02675-0

    Figure Lengend Snippet: Fig. 3 Evaluation of ROS levels in HeLa and Ca Ski cells following pCMV6-ATF3 transfection. (A) Flow cytometry histograms illustrating ROS levels in HeLa and Ca Ski cells, measured with the DCHF-DA assay. (B) Quantitative comparison shows a significant rise in ROS levels in pCMV6-ATF3-transfected cells relative to mock and control groups (p < 0.01). Results are shown as the mean ± SD from three separate experiments

    Article Snippet: ATF3 protein levels were detected using a monoclonal anti-ATF3 antibody (1:500, Santa Cruz Biotechnologies). β-actin (1:5000, Santa Cruz Biotechnologies) was used as a loading control.

    Techniques: Transfection, Flow Cytometry, Comparison, Control

    Fig. 4 DNA damage analysis by comet assay using alkaline single-cell gel electrophoresis. (A) Representative images from the comet assay display DNA migration patterns in HeLa and Ca Ski cells post-transfection with pCMV6-ATF3. In these images, intact DNA forms a distinct head, while fragmented DNA trails behind, forming a comet tail—longer tails signify greater DNA damage. (B) Quantification of comet assay results for HeLa and Ca Ski cells across pCMV6-ATF3-transfected, mock-transfected, and control groups. Minimal DNA damage was observed in both control and mock-transfected cells. Data are expressed as the mean ± SD from three independent replicates

    Journal: Virology journal

    Article Title: Activating transcription factor 3 induces oxidative stress and genotoxicity, transcriptionally modulating metastasis-related gene expression in human papillomavirus-infected cervical cancer.

    doi: 10.1186/s12985-025-02675-0

    Figure Lengend Snippet: Fig. 4 DNA damage analysis by comet assay using alkaline single-cell gel electrophoresis. (A) Representative images from the comet assay display DNA migration patterns in HeLa and Ca Ski cells post-transfection with pCMV6-ATF3. In these images, intact DNA forms a distinct head, while fragmented DNA trails behind, forming a comet tail—longer tails signify greater DNA damage. (B) Quantification of comet assay results for HeLa and Ca Ski cells across pCMV6-ATF3-transfected, mock-transfected, and control groups. Minimal DNA damage was observed in both control and mock-transfected cells. Data are expressed as the mean ± SD from three independent replicates

    Article Snippet: ATF3 protein levels were detected using a monoclonal anti-ATF3 antibody (1:500, Santa Cruz Biotechnologies). β-actin (1:5000, Santa Cruz Biotechnologies) was used as a loading control.

    Techniques: Single Cell Gel Electrophoresis, Alkaline Single Cell Gel Electrophoresis, Migration, Transfection, Control